Day 1:
8-9A Registration
9-11A: Morning session
Intro to course
Presentation: Microscopy in Biology
10A Break
Lecture: Microscope optics, NA, light throughput (chalk)
Lunch 11-1P
1-3P: Afternoon session
Lecture: Fluorescence microscopy (chalk)
Presentation: Fluorescence Microscopy
3-5: Lab
Disassemble scopes, hardware, bulbs, alignment, filters, beginning sample prep, demo fluorescence microscopes. Also, single vs multi-color filter sets, sample preparation and storage, immersion media

Day 2
8:30-10: Morning session
Lecture: Confocal microscopy (chalk)
10A Break
10:15-11:30
Presentation: Intro to Confocal microscopy
Lunch: 11:30-1P
1-1:30: Finish Intro presentation
2-5: Lab: confocal hardware, beginning confocal imaging, detectors, pinhole, 1, 2, and 3-channel scanning. multiple laser excitation, MultiTracking

Day 3
8:30-9:30
Presentation: Confocal Considerations
Break
10:15-11:00 Presentation: Lambda Scanning, Linear Unmixing, FRAP
11:30-1PM: Lunch
1-4: Lab: FRAP, FRET, Lambda Scans, Linear unmixing
4-5: Presentation: Golub Talk

Day 4
8:30 - 9:30: Presentation: Digital Imaging
11A: Q&A session

Useful websites:

• ImageJ: Free image processing software
• Steve's Techniques in Light Microscopy course