Alkaline Phosphatase localization in the aleurone layer of barley. Imbibed (24h) kernel was sectiones at 15µm in a cryotome. The section was mounted to a glass slide and flooded with NBT/BCIP solution. Within a few minutes a blue ppt formed, indicating the presence of AlkPhos.

This project was done in Plant & Animal Microtechnique., a one-week workshop at UC Berkeley.